Specific electrochemical phage sensing for Bacillus cereus and Mycobacterium smegmatis

Miri Yemini; Yaron Levi; Ezra Yagil; Judith Rishpon

doi:10.1016/j.bioelechem.2006.03.014

Specific electrochemical phage sensing for Bacillus cereus and Mycobacterium smegmatis

Miri Yemini, Yaron Levi, Ezra Yagil, Judith Rishpon^*

^*Corresponding author for this work

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The rapid and reliable detection of pathogenic microorganisms is an important issue for the safety and security of our society. Here we describe the use of a sensitive, inexpensive, amperometric, phage-based biosensor for the detection of extremely low concentrations of Bacillus cereus and Mycobacterium smegmatis as models for Bacillus anthracis (the causative agent of anthrax) and for Mycobacterium tuberculosis (the causative agent of tuberculosis), respectively. The detection procedure developed here enabled the determination of bacteria at a low concentration of 10 viable cells/mL within 8 h. This experimental setup allows the simultaneous analysis of up to eight independent samples, using disposable screen-printed electrodes.

Original language	English
Pages (from-to)	180-184
Number of pages	5
Journal	Bioelectrochemistry
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/j.bioelechem.2006.03.014
State	Published - Jan 2007

Keywords

Anthrax
Bacteriophage
Detection
Electrochemical
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bioelechem.2006.03.014

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abstract = "The rapid and reliable detection of pathogenic microorganisms is an important issue for the safety and security of our society. Here we describe the use of a sensitive, inexpensive, amperometric, phage-based biosensor for the detection of extremely low concentrations of Bacillus cereus and Mycobacterium smegmatis as models for Bacillus anthracis (the causative agent of anthrax) and for Mycobacterium tuberculosis (the causative agent of tuberculosis), respectively. The detection procedure developed here enabled the determination of bacteria at a low concentration of 10 viable cells/mL within 8 h. This experimental setup allows the simultaneous analysis of up to eight independent samples, using disposable screen-printed electrodes.",

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AB - The rapid and reliable detection of pathogenic microorganisms is an important issue for the safety and security of our society. Here we describe the use of a sensitive, inexpensive, amperometric, phage-based biosensor for the detection of extremely low concentrations of Bacillus cereus and Mycobacterium smegmatis as models for Bacillus anthracis (the causative agent of anthrax) and for Mycobacterium tuberculosis (the causative agent of tuberculosis), respectively. The detection procedure developed here enabled the determination of bacteria at a low concentration of 10 viable cells/mL within 8 h. This experimental setup allows the simultaneous analysis of up to eight independent samples, using disposable screen-printed electrodes.

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KW - Bacteriophage

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KW - Electrochemical

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Specific electrochemical phage sensing for Bacillus cereus and Mycobacterium smegmatis

Abstract

Keywords

UN SDGs

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