Specific Desulfuromonas strains can determine startup times of microbial fuel cells

Keren Yanuka-Golub*, Leah Reshef, Judith Rishpon, Uri Gophna

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Microbial fuel cells (MFCs) can generate electricity simultaneously with wastewater treatment. For MFCs to be considered a cost-effective treatment technology, they should quickly re-establish a stable electroactive microbial community in the case of system failure. In order to shorten startup times, temporal studies of anodic biofilm development are required, however, frequent sampling can reduce the functionality of the system due to electroactive biomass loss; therefore, on-line monitoring of the microbial community without interfering with the system’s stability is essential. Although all anodic biofilms were composed of Desulfuromonadaceae, MFCs differed in startup times. Generally, a Desulfuromonadaceae-dominated biofilm was associated with faster startup MFCs. A positive PCR product of a specific 16S rRNA gene PCR primer set for detecting the acetate-oxidizing, Eticyclidine (PCE)-dechlorinating Desulfuromonas group was associated with efficient MFCs in our samples. Therefore, this observation could serve as a biomarker for monitoring the formation of an efficient anodic biofilm. Additionally, we successfully enriched an electroactive consortium from an active anode, also resulting in a positive amplification of the specific primer set. Direct application of this enrichment to a clean MFC anode showed a substantial reduction of startup times from 18 to 3 days.

Original languageEnglish
Article number8570
Pages (from-to)1-12
Number of pages12
JournalApplied Sciences (Switzerland)
Volume10
Issue number23
DOIs
StatePublished - 1 Dec 2020

Funding

FundersFunder number
Israel?s Ministry of Environmental Protection
Israel’s Ministry of Environmental Protection
MOEP132-4-2
Sackler Faculty of Medicine, Tel-Aviv University

    Keywords

    • Anodic biofilm
    • Desulfuromonadaceae
    • Microbial fuel cells (MFCs)
    • Specific 16S-Desulfuromonas primer set
    • Startup time

    Fingerprint

    Dive into the research topics of 'Specific Desulfuromonas strains can determine startup times of microbial fuel cells'. Together they form a unique fingerprint.

    Cite this