Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA

Ilana Lotan*, Philip Goelet, Ariela Gigi, Nathan Dascal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Although the structure of rabbit skeletal muscle dihydropyridine (DHP) receptor, deduced from cDNA sequence, indicates that this protein is the channel-forming subunit of voltage-dependent calcium channel (VDCC), no functional proof for this prediction has been presented. Two DNA oligonucleotides complementary to DHP-receptor RNA sequences coding for putative membrane-spanning regions of the DHP receptor specifically suppress the expression of the DHP-sensitive VDCC from rabbit and rat heart in Xenopus oocytes. However, these oligonucleotides do not suppress the expression of the DHP-insensitive VDCC and of voltage-dependent sodium and potassium channels. Thus, the gene for DHP receptor of rabbit skeletal muscle is closely related, or identical to, a gene expressed in heart that encodes a component of the DHP-sensitive VDCC The DHP-sensitive and DHP-insensitive VDCCs are distinct molecular entities.

Original languageEnglish
Pages (from-to)666-669
Number of pages4
JournalScience
Volume243
Issue number4891
DOIs
StatePublished - 1989

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