Specific Antigrowth Effect of Interferon on Mouse Cells Transformed by Murine Sarcoma Virus

The growth rate of NIH/3T3 mouse fibroblasts transformed by the Moloney strain of murine sarcoma virus was investigated following interferon (IFN) treatment. These cells were found to be sensitive to the antigrowth effect of IFN as indicated by a slower growth rate in its presence. The effect was most efficiently expressed when cells were grown at low serum concentrations, e.g., 2.5%. Likewise, IFN treatment caused a reduction in the rate of DNA synthesis as measured by [³H]thymidine incorporation. In contrast, these effects were observed only to a very minor extent with uninfected NIH/3T3 cells or with NIH/3T3 cells chronically infected with murine leukemia virus. In addition, IFN treatment significantly decreased the cloning efficiency of murine sarcoma virus-transformed cells in semisolid agar. Furthermore, an even stronger effect on the cloning efficiency was observed in liquid medium supplied with 2.5% serum, indicating a direct inhibitory effect on the growth of these cells. Under these conditions, NIH/3T3 or NIH/3T3(MLV) cells remained unaffected by IFN treatment, nor was this effect evident when the transformed cells were grown in the presence of 10% serum. A 4-fold increase in the level of (2'^-5)oligoadenylate synthetase following IFN treatment was observed in murine sarcoma virus-transformed cells as compared to either NIH/3T3 or NIH/3T3(MLV) cells.