@article{d33263bd0fe14674a64840c1ae453721,
title = "Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC",
abstract = "We present a technique that records transient changes in the fluorescence lifetime of a sample with spatial resolution along a one-dimensional scan. The technique is based on scanning the sample with a high-frequency pulsed laser beam, detecting single photons of the fluorescence light, and building up a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a stimulation of the sample. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about one millisecond. We demonstrate the technique for recording photochemical and nonphotochemical chlorophyll transients in plants and transient changes in free Ca2+ in cultured neurons. Microsc. Res. Tech. 77:216-224, 2014.",
keywords = "Chlorophyll transients, FLIM, FLITS, Fluorescence lifetime, TCSPC",
author = "Wolfgang Becker and Vladislav Shcheslavkiy and Samuel Frere and Inna Slutsky",
year = "2014",
month = mar,
doi = "10.1002/jemt.22331",
language = "אנגלית",
volume = "77",
pages = "216--224",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "Wiley-Liss Inc.",
number = "3",
}