TY - JOUR
T1 - Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy
AU - Rappaz, Benjamin
AU - Barbul, Alexander
AU - Hoffmann, Annick
AU - Boss, Daniel
AU - Korenstein, Rafi
AU - Depeursinge, Christian
AU - Magistretti, Pierre J.
AU - Marquet, Pierre
N1 - Funding Information:
The authors would like to thanks Corinne Moratal for her technical assistance and the personnel of Lyncée Tec SA ( www.lynceetec.com ), PSE-A, CH-1015 Lausanne, Switzerland, for their technical support and the fruitful discussions during the course of the experimentation. This work has been supported by the Swiss National Science Foundation (grant 205320-112195/1) and CTI project 9389.1. A.B. has been supported by Nano2life European Network of Excellence travel grants. Aspects of this article were presented in the 2008 Red Cell Conference held at the University of Rochester, New York, in memory of Philip Knauf.
PY - 2009/5
Y1 - 2009/5
N2 - Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.
AB - Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 ± 8.9 nm and 4.7 ± 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.
KW - Cell membrane fluctuations
KW - Digital holography
KW - Quantitative phase microscopy
KW - Red blood cell
KW - Refractive index
UR - http://www.scopus.com/inward/record.url?scp=64849114184&partnerID=8YFLogxK
U2 - 10.1016/j.bcmd.2009.01.018
DO - 10.1016/j.bcmd.2009.01.018
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C2 - 19324576
AN - SCOPUS:64849114184
SN - 1079-9796
VL - 42
SP - 228
EP - 232
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
IS - 3
ER -