Some regulatory properties of purine biosynthesis de novo in long-term cultures of epithelial-like rat liver cells

Pnina Bashkin*, Oded Sperling

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

De novo synthesis of purine nucleotides and some regulatory properties of this pathway were studied in cultured epithelial-like rat liver cells. It was found that the physiological 5-phosphoribosyl 1-pyrophosphate (P-Rib-PP) concentration in these cells is limiting for purine synthesis de novo. Increase of P-Rib-PP availability, achieved by activation of P-Rib-PP synthetase at high Pi concentration, resulted in acceleration of purine synthesis. The effects of increasing cellular ribose 5-phosphate (Rib-5-P availability, by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on P-Rib-PP availability and on the rate of the novo purine synthesis were also studied. It was found that at the Pi concentration prevailing in the tissue at extracellular physiological Pi concentration, Rib-5-P availability is saturating for P-Rib-PP generation and therefore also for purine synthesis.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume538
Issue number3
DOIs
StatePublished - 1 Feb 1978

Funding

FundersFunder number
USA-Israel Binational Science Foundation
Ministry of Health, State of Israel

    Fingerprint

    Dive into the research topics of 'Some regulatory properties of purine biosynthesis de novo in long-term cultures of epithelial-like rat liver cells'. Together they form a unique fingerprint.

    Cite this