Some molecular properties of an isolated acetylcholine receptor ion-translocation protein

D. Michaelson; R. Vandlen; J. Bode; T. Moody; J. Schmidt; M. A. Raftery

doi:10.1016/0003-9861(74)90309-9

Some molecular properties of an isolated acetylcholine receptor ion-translocation protein

D. Michaelson, R. Vandlen, J. Bode, T. Moody, J. Schmidt, M. A. Raftery

Research output: Contribution to journal › Article › peer-review

Abstract

An improved procedure for isolation and purification of acetylcholine receptor from Torpedo californica electroplax membranes is described. The purified material contains the neurotransmitter recognition site and a second binding subsite which complexes inorganic cations and bis-quaternary cholinergic analogs. In addition to the transmitter recognition site the isolated macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified acetylcholine receptor and Torpedo phospholipids.

Original language	English
Pages (from-to)	796-804
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	165
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(74)90309-9
State	Published - Dec 1974
Externally published	Yes

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10.1016/0003-9861(74)90309-9

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title = "Some molecular properties of an isolated acetylcholine receptor ion-translocation protein",

abstract = "An improved procedure for isolation and purification of acetylcholine receptor from Torpedo californica electroplax membranes is described. The purified material contains the neurotransmitter recognition site and a second binding subsite which complexes inorganic cations and bis-quaternary cholinergic analogs. In addition to the transmitter recognition site the isolated macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified acetylcholine receptor and Torpedo phospholipids.",

author = "D. Michaelson and R. Vandlen and J. Bode and T. Moody and J. Schmidt and Raftery, {M. A.}",

note = "Funding Information: {\textquoteleft}This research was supported by USPHS Grant NS 10294. ZA recipient of a fellowship from the Deutsche Forschungsgemeinschaft and MAR of a USPHS Research Career Development Award. {\textquoteright} Contribution No. 4952. {\textquoteright} Abbreviations used are cu-Bgt, oc-bungarotoxin; AcChR, acetylcholine receptor; DAP, bis-[3-aminopyridinium] 1, lo-decane diiodide.",

year = "1974",

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doi = "10.1016/0003-9861(74)90309-9",

language = "אנגלית",

volume = "165",

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issn = "0003-9861",

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T1 - Some molecular properties of an isolated acetylcholine receptor ion-translocation protein

AU - Michaelson, D.

AU - Vandlen, R.

AU - Bode, J.

AU - Moody, T.

AU - Schmidt, J.

AU - Raftery, M. A.

N1 - Funding Information: ‘This research was supported by USPHS Grant NS 10294. ZA recipient of a fellowship from the Deutsche Forschungsgemeinschaft and MAR of a USPHS Research Career Development Award. ’ Contribution No. 4952. ’ Abbreviations used are cu-Bgt, oc-bungarotoxin; AcChR, acetylcholine receptor; DAP, bis-[3-aminopyridinium] 1, lo-decane diiodide.

PY - 1974/12

Y1 - 1974/12

N2 - An improved procedure for isolation and purification of acetylcholine receptor from Torpedo californica electroplax membranes is described. The purified material contains the neurotransmitter recognition site and a second binding subsite which complexes inorganic cations and bis-quaternary cholinergic analogs. In addition to the transmitter recognition site the isolated macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified acetylcholine receptor and Torpedo phospholipids.

AB - An improved procedure for isolation and purification of acetylcholine receptor from Torpedo californica electroplax membranes is described. The purified material contains the neurotransmitter recognition site and a second binding subsite which complexes inorganic cations and bis-quaternary cholinergic analogs. In addition to the transmitter recognition site the isolated macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified acetylcholine receptor and Torpedo phospholipids.

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JO - Archives of Biochemistry and Biophysics

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SN - 0003-9861

IS - 2

ER -

Some molecular properties of an isolated acetylcholine receptor ion-translocation protein

Abstract

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