Somatostatin inhibits pro-inflammatory cytokine secretion from rat hepatic stellate cells

Alon Lang, Emad Sakhnini, Herma H. Fidder, Yaakov Maor, Simon Bar-Meir, Yehuda Chowers

Research output: Contribution to journalArticlepeer-review

Abstract

Background/aims: Activated hepatic stellate cells (HSCs) have been implicated in hepatic fibrosis. Somatostatin (SOM) has an immunomodulatory role. The aim of this study was to assess the secretion of pro-inflammatory cytokines by HSCs and to determine the effect of SOM on the secretion of these mediators. Methods: Activated rat HSCs were evaluated for their secretion of IL-1β, IL-8, and TNF-α using ELISA. RNA protection assay was used to determine cytokine mRNA levels. The expression of chemokine and cytokine mRNA and the secretion of these mediators were assessed following incubation with SOM or octreotide. Results: HSCs spontaneously secreted IL-1β, IL-8, and TNF-α. This secretion was augmented following stimulation by IL-1β and TNF-α. SOM inhibited the spontaneous and TNF-α-induced secretion of IL-1β, IL-8, and TNF-α and suppressed the expression of IL-1β and TNF-α mRNA. Octreotide suppressed the secretion of IL-1β and IL-8. Conclusions: These observations indicate that SOM exerts an inhibitory immunomodulatory effect on HSCs.

Original languageEnglish
Pages (from-to)808-816
Number of pages9
JournalLiver International
Volume25
Issue number4
DOIs
StatePublished - Aug 2005
Externally publishedYes

Keywords

  • Chemokines
  • Cytokines
  • Hepatic stellate cells
  • Inflammation
  • Liver fibrosis
  • Somatostatin

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