TY - JOUR
T1 - Somatic cell hybridization between bovine leukemia virus-infected lymphocytes and murine plasma cell tumors
T2 - Cell fusion studies with bovine cells
AU - Davidson, Irit
AU - Katzav, Shulamit
AU - Ungar-Waron, Hanna
AU - Eshhar, Zelig
AU - Haimovich, Joseph
AU - Trainin, Ze'ev
N1 - Funding Information:
* This research was supported by grant No. 1664 from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel, and the Leukemia Research Foundation, Chicago. Illinois, U.S.A. /)T o whom correspondence should be addressed. f Abbreviations: BLV, bovine leukemia virus; DMEM, Dulbecco’s modified Eagle’s medium; HAT, hypoxanthine, aminopterin and thymidine; HS, horse serum; Ig, immunoglobulin; mIgM. membrane IgM; ME, 2-mercaptoethanol; sIgM, secreted IgM; SDS, sodium dodecyl sulphate; PAGE, polyacrylamide gel electrophoresis; PBL, peripheral blood lymphocytes.
PY - 1982/7
Y1 - 1982/7
N2 - Hybridization of peripheral blood lymphocytes from bovine leukemia virus-infected cows with murine myeloma cells resulted in the generation of hybrid cells secreting immunoglobulins composed of various combinations of heavy and light chains of both bovine and murine Ig origin. Some hybrid cells derived from the light-chain producer, but non-secretor murine myeloma NS1 cell line, secreted IgM molecules composed of bovine μ-chain linked to bovine and/or murine light chains. Other hybridomas secreted mouse and bovine light-chain dimers and/or monomers, or failed to secrete any Ig polypeptide chain whatsoever. Immunoglobulins secreted by hybridomas obtained upon hybridization of bovine cells with the IgG-secreting murine myeloma P3X63 cell line, contained bovine μ-chain in one of the seven hybridomas obtained, and bovine light chain in two of them. All the cell lines secreted murine light- and γ-chains.
AB - Hybridization of peripheral blood lymphocytes from bovine leukemia virus-infected cows with murine myeloma cells resulted in the generation of hybrid cells secreting immunoglobulins composed of various combinations of heavy and light chains of both bovine and murine Ig origin. Some hybrid cells derived from the light-chain producer, but non-secretor murine myeloma NS1 cell line, secreted IgM molecules composed of bovine μ-chain linked to bovine and/or murine light chains. Other hybridomas secreted mouse and bovine light-chain dimers and/or monomers, or failed to secrete any Ig polypeptide chain whatsoever. Immunoglobulins secreted by hybridomas obtained upon hybridization of bovine cells with the IgG-secreting murine myeloma P3X63 cell line, contained bovine μ-chain in one of the seven hybridomas obtained, and bovine light chain in two of them. All the cell lines secreted murine light- and γ-chains.
UR - http://www.scopus.com/inward/record.url?scp=0019992448&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(82)90355-8
DO - 10.1016/0161-5890(82)90355-8
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AN - SCOPUS:0019992448
SN - 0161-5890
VL - 19
SP - 893
EP - 899
JO - Molecular Immunology
JF - Molecular Immunology
IS - 7
ER -
