Somatic cell hybridization between bovine leukemia virus-infected lymphocytes and murine plasma cell tumors: Cell fusion studies with bovine cells

Irit Davidson, Shulamit Katzav, Hanna Ungar-Waron, Zelig Eshhar, Joseph Haimovich, Ze'ev Trainin

Research output: Contribution to journalArticlepeer-review

Abstract

Hybridization of peripheral blood lymphocytes from bovine leukemia virus-infected cows with murine myeloma cells resulted in the generation of hybrid cells secreting immunoglobulins composed of various combinations of heavy and light chains of both bovine and murine Ig origin. Some hybrid cells derived from the light-chain producer, but non-secretor murine myeloma NS1 cell line, secreted IgM molecules composed of bovine μ-chain linked to bovine and/or murine light chains. Other hybridomas secreted mouse and bovine light-chain dimers and/or monomers, or failed to secrete any Ig polypeptide chain whatsoever. Immunoglobulins secreted by hybridomas obtained upon hybridization of bovine cells with the IgG-secreting murine myeloma P3X63 cell line, contained bovine μ-chain in one of the seven hybridomas obtained, and bovine light chain in two of them. All the cell lines secreted murine light- and γ-chains.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalMolecular Immunology
Volume19
Issue number7
DOIs
StatePublished - Jul 1982

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