Somatic cell genetics and flow cytometry

Michael E. Kamarck; James A. Barbosa; Lukas Kühn; Pamela G.Messer Peters; Lester Shulman; Frank H. Ruddle

doi:10.1002/cyto.990040202

Somatic cell genetics and flow cytometry

Michael E. Kamarck^*, James A. Barbosa, Lukas Kühn, Pamela G.Messer Peters, Lester Shulman, Frank H. Ruddle

^*Corresponding author for this work

Yale University

Research output: Contribution to journal › Review article › peer-review

9 Scopus citations

Abstract

Human genes coding cell surface molecules can be introduced into mouse host cells using a variety of somatic cell genetic techniques. Because these human gene products can be detected using indirect immunofluorescene on viable cells, the genes themsleves can be monitored and manipulated using flow cytometry and sorting. In this paper, we review ways that we have used cell sorting to develop a somatic cell genetic analysis of the human cell surface.

Original language	English
Pages (from-to)	99-108
Number of pages	10
Journal	Cytometry
Volume	4
Issue number	2
DOIs	https://doi.org/10.1002/cyto.990040202
State	Published - Sep 1983
Externally published	Yes

Keywords

DNA‐mediated gene transfer
Somatic cells hybrids
cell sorting
cell surface antigens
flow cytometry

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10.1002/cyto.990040202

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keywords = "DNA‐mediated gene transfer, Somatic cells hybrids, cell sorting, cell surface antigens, flow cytometry",

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year = "1983",

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AU - Barbosa, James A.

AU - Kühn, Lukas

AU - Peters, Pamela G.Messer

AU - Shulman, Lester

AU - Ruddle, Frank H.

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AB - Human genes coding cell surface molecules can be introduced into mouse host cells using a variety of somatic cell genetic techniques. Because these human gene products can be detected using indirect immunofluorescene on viable cells, the genes themsleves can be monitored and manipulated using flow cytometry and sorting. In this paper, we review ways that we have used cell sorting to develop a somatic cell genetic analysis of the human cell surface.

KW - DNA‐mediated gene transfer

KW - Somatic cells hybrids

KW - cell sorting

KW - cell surface antigens

KW - flow cytometry

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Somatic cell genetics and flow cytometry

Abstract

Keywords

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