Soluble P-selectin and the postoperative course following cardiopulmonary bypass in children

D. Lotan, T. Prince, O. Dagan, N. Keller, R. Ben-Abraham, A. Weinbroum, A. Gaby, A. Augarten, A. Smolinski, Z. Barzilay, G. Paret

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cytokine-inducible leucocyte-endothelial adhesion molecules were shown to affect the postoperative inflammatory response following cardiopulmonary bypass (CPB). Soluble P-selectin (sP-selectin) is one of these molecules. We investigated the correlation between plasma sP-selectin levels and the intra- and postoperative course in children undergoing CPB. Methods: Serial blood samples of 13 patients were collected preoperatively upon initiation of CPB and seven times postoperatively. Plasma was recovered immediately and frozen at - 70°C until use. Circulating soluble selectin molecules were measured with a sandwich enzyme-linked immunoabsorbent assay technique. Results: The significant post-CPB changes in sP-selectins plasma levels were associated with patient characteristics, operative variables and postoperative course. sP-selectin levels correlated significantly with surgery time, aortic cross-clamping time and inotropic support, as well as with the postoperative Pediatric Risk of Mortality score, hypotension and tachycardia. Conclusions: A relation between CPB-induced mediators and both early and late clinical effects is suggested. The up-regulation and expression of sP-selectin indicate neutrophil activation as a possible mechanism for the increase, and inhibiting it may reduce the inflammatory response associated with CPB.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalPaediatric Anaesthesia
Volume11
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Adhesion molecules
  • Cardiopulmonary bypass
  • Inflammatory reaction
  • Neutrophils
  • sP-selectin

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