TY - JOUR
T1 - Soluble and matrix-associated heparan sulfate proteoglycans increase expression of erb-b2 and erb-b3 in colon cancer cell lines
AU - Zvibel, Isabel
AU - Brill, Shlomo
AU - Halpern, Zamir
AU - Moskovitz, Sharon
AU - Yayon, Avner
AU - Papa, Moshe
PY - 2001/2/1
Y1 - 2001/2/1
N2 - We investigated the effect of hepatocyte-derived extracellular matrix (ECM) on the expression of erb-B2 and erb-B3 in colon cancer cell lines, as well as the role of erb-B2 and erb-B3 in colon cancer cell proliferation. Colon cancer cell lines plated on hepatocyte-derived ECM had increased protein levels of both erb-B2 and erb-B3. The addition of soluble recombinant proteoglycan syndecan-4 also resulted in higher expression of erb-B2 and erb-B3. We prepared hepatocyte-derived ECM from 1 to 7 days' cultures of hepatocytes, which contained different amounts of sulfated glycosaminoglycans. There was a direct correlation between the amounts of sulfated glycosaminoglycans in the ECM and the levels of erb-B2 and erb-B3 in the colon cell line KM12. The stimulatory effect of hepatocyte-derived ECM was abolished when the colon cancer cells were cultured in the presence of antibodies to erb-B2. These studies show that hepatocyte-derived ECM and the heparan sulfate proteoglycans present in it are responsible for inducing erb-B2 and erb-B3 in colon cancer cells. The growth stimulatory effect of extracellular matrix is mediated, at least in part, by increased expression of erb-B2 and erb-B3.
AB - We investigated the effect of hepatocyte-derived extracellular matrix (ECM) on the expression of erb-B2 and erb-B3 in colon cancer cell lines, as well as the role of erb-B2 and erb-B3 in colon cancer cell proliferation. Colon cancer cell lines plated on hepatocyte-derived ECM had increased protein levels of both erb-B2 and erb-B3. The addition of soluble recombinant proteoglycan syndecan-4 also resulted in higher expression of erb-B2 and erb-B3. We prepared hepatocyte-derived ECM from 1 to 7 days' cultures of hepatocytes, which contained different amounts of sulfated glycosaminoglycans. There was a direct correlation between the amounts of sulfated glycosaminoglycans in the ECM and the levels of erb-B2 and erb-B3 in the colon cell line KM12. The stimulatory effect of hepatocyte-derived ECM was abolished when the colon cancer cells were cultured in the presence of antibodies to erb-B2. These studies show that hepatocyte-derived ECM and the heparan sulfate proteoglycans present in it are responsible for inducing erb-B2 and erb-B3 in colon cancer cells. The growth stimulatory effect of extracellular matrix is mediated, at least in part, by increased expression of erb-B2 and erb-B3.
KW - Colon cancer
KW - Extracellular matrix
KW - Heparan sulfate
UR - http://www.scopus.com/inward/record.url?scp=0035254952&partnerID=8YFLogxK
U2 - 10.1002/1097-0215(200002)9999:9999<::AID-IJC1045>3.0.CO;2-O
DO - 10.1002/1097-0215(200002)9999:9999<::AID-IJC1045>3.0.CO;2-O
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0035254952
SN - 0020-7136
VL - 91
SP - 316
EP - 321
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 3
ER -