Soluble and matrix-associated heparan sulfate proteoglycans increase expression of erb-b2 and erb-b3 in colon cancer cell lines

Isabel Zvibel*, Shlomo Brill, Zamir Halpern, Sharon Moskovitz, Avner Yayon, Moshe Papa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We investigated the effect of hepatocyte-derived extracellular matrix (ECM) on the expression of erb-B2 and erb-B3 in colon cancer cell lines, as well as the role of erb-B2 and erb-B3 in colon cancer cell proliferation. Colon cancer cell lines plated on hepatocyte-derived ECM had increased protein levels of both erb-B2 and erb-B3. The addition of soluble recombinant proteoglycan syndecan-4 also resulted in higher expression of erb-B2 and erb-B3. We prepared hepatocyte-derived ECM from 1 to 7 days' cultures of hepatocytes, which contained different amounts of sulfated glycosaminoglycans. There was a direct correlation between the amounts of sulfated glycosaminoglycans in the ECM and the levels of erb-B2 and erb-B3 in the colon cell line KM12. The stimulatory effect of hepatocyte-derived ECM was abolished when the colon cancer cells were cultured in the presence of antibodies to erb-B2. These studies show that hepatocyte-derived ECM and the heparan sulfate proteoglycans present in it are responsible for inducing erb-B2 and erb-B3 in colon cancer cells. The growth stimulatory effect of extracellular matrix is mediated, at least in part, by increased expression of erb-B2 and erb-B3.

Original languageEnglish
Pages (from-to)316-321
Number of pages6
JournalInternational Journal of Cancer
Volume91
Issue number3
DOIs
StatePublished - 1 Feb 2001
Externally publishedYes

Keywords

  • Colon cancer
  • Extracellular matrix
  • Heparan sulfate

Fingerprint

Dive into the research topics of 'Soluble and matrix-associated heparan sulfate proteoglycans increase expression of erb-b2 and erb-b3 in colon cancer cell lines'. Together they form a unique fingerprint.

Cite this