TY - JOUR
T1 - Solubilization of phospholipids by detergents structural and kinetic aspects
AU - Lichtenberg, Dov
AU - Robson, Robert J.
AU - Dennis, Edward A.
N1 - Funding Information:
We wish to thank the National Science Foundation for support of this work under grant PCM 82-16963. Critical suggestions and stimulating discussions on solubilization with Dr. Karol J. Mysels and Professor Alan F. Hofmann at the University
PY - 1983/5/24
Y1 - 1983/5/24
N2 - Most amphiphiles in biological membranes including phospholipids, steroids, and membrane proteins are insoluble amphiphiles and would form liquid crystals or insoluble precipitates alone in aqueous media. Detergents are soluble amphiphiles and above a critical concentration and temperature form micelles of various sizes and shapes. Much of the recent progress in studying the insoluble amphiphiles is due to the formation of thermodynamically stable isotropic solutions of these compounds in the presence of detergents. This process, which is commonly denoted as 'solubilization,', involves transformation of lamellar structures into mixed micelles. The information available to date on the solubilization of phospholipids, which constitute the lipid skeleton of biomembranes, by the common detergents is discussed in this review, both with respect to the kinetics of this process and the structure of the various phospholipid-detergent mixed micelles formed. It is hoped that this discussion will lead to somewhat more useful, although still necessarily fairly empirical, approaches to the solubilization of phospholipids by detergents.
AB - Most amphiphiles in biological membranes including phospholipids, steroids, and membrane proteins are insoluble amphiphiles and would form liquid crystals or insoluble precipitates alone in aqueous media. Detergents are soluble amphiphiles and above a critical concentration and temperature form micelles of various sizes and shapes. Much of the recent progress in studying the insoluble amphiphiles is due to the formation of thermodynamically stable isotropic solutions of these compounds in the presence of detergents. This process, which is commonly denoted as 'solubilization,', involves transformation of lamellar structures into mixed micelles. The information available to date on the solubilization of phospholipids, which constitute the lipid skeleton of biomembranes, by the common detergents is discussed in this review, both with respect to the kinetics of this process and the structure of the various phospholipid-detergent mixed micelles formed. It is hoped that this discussion will lead to somewhat more useful, although still necessarily fairly empirical, approaches to the solubilization of phospholipids by detergents.
UR - http://www.scopus.com/inward/record.url?scp=0021111031&partnerID=8YFLogxK
U2 - 10.1016/0304-4157(83)90004-7
DO - 10.1016/0304-4157(83)90004-7
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AN - SCOPUS:0021111031
SN - 0304-4157
VL - 737
SP - 285
EP - 304
JO - BBA - Reviews on Biomembranes
JF - BBA - Reviews on Biomembranes
IS - 2
ER -