TY - JOUR
T1 - Sodium Ion Transport in Rat Hearts during Cold Ischemic Storage
T2 - 23Na and 31P NMR Study
AU - Askenasy, Nadir
AU - Vivi, Antonio
AU - Tassini, Maria
AU - Navon, Gil
PY - 1992/12
Y1 - 1992/12
N2 - The success of heart transplantation is limited by the negative correlation between the length of the cold ischemic storage period and the quality of functional recovery. We use 23Na, 31P NMR spectroscopy, and hemodynamic parameters to describe temperature‐dependent changes in sodium influx and the concentration of phosphorus high‐energy compounds during different storage periods. Perfusion with Krebs—Henseleit solutions containing Dy(TTHA)3‐ permitted discrimination of intra‐ and extracellular sodium during cold ischemic storage. The 23Na NMR visibilities under the acquisition and processing parameters used in our experiments were 40 ± 4% for the intracellular compartment and 97 ± 11% for the extracellular compartment. At 4°C, the intracellular Na+ accumulation exceeded that observed at 15 and 22°C. The ATP and PCr depletion rates were much lower at 4°C and the left ventricular contractility was higher after longer periods of storage, as the storage temperature decreased. The intracellular Na+ concentration cannot serve as a marker for the postischemic recovery probability. The relative activity of the Na/K ATPase pumps is not correlated with the preservation success. However, intracellular sodium ion accumulation is a major factor in the time lag of the reperfusion recovery.© 1992 Academic Press,Inc.
AB - The success of heart transplantation is limited by the negative correlation between the length of the cold ischemic storage period and the quality of functional recovery. We use 23Na, 31P NMR spectroscopy, and hemodynamic parameters to describe temperature‐dependent changes in sodium influx and the concentration of phosphorus high‐energy compounds during different storage periods. Perfusion with Krebs—Henseleit solutions containing Dy(TTHA)3‐ permitted discrimination of intra‐ and extracellular sodium during cold ischemic storage. The 23Na NMR visibilities under the acquisition and processing parameters used in our experiments were 40 ± 4% for the intracellular compartment and 97 ± 11% for the extracellular compartment. At 4°C, the intracellular Na+ accumulation exceeded that observed at 15 and 22°C. The ATP and PCr depletion rates were much lower at 4°C and the left ventricular contractility was higher after longer periods of storage, as the storage temperature decreased. The intracellular Na+ concentration cannot serve as a marker for the postischemic recovery probability. The relative activity of the Na/K ATPase pumps is not correlated with the preservation success. However, intracellular sodium ion accumulation is a major factor in the time lag of the reperfusion recovery.© 1992 Academic Press,Inc.
UR - http://www.scopus.com/inward/record.url?scp=0027104316&partnerID=8YFLogxK
U2 - 10.1002/mrm.1910280207
DO - 10.1002/mrm.1910280207
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0027104316
SN - 0740-3194
VL - 28
SP - 249
EP - 263
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
IS - 2
ER -