TY - JOUR
T1 - SNV, a lipophilic superactive VIP analog, acts through cGMP to promote neuronal survival
AU - Ashur-Fabian, Osnat
AU - Perl, Orly
AU - Lilling, Gila
AU - Fridkin, Mati
AU - Gozes, Illana
N1 - Funding Information:
Supported, in part, by the US–Israel Binational Science Foundation. Prof. Illana Gozes is the incumbent of the Lily and Avraham Gildor Chair for the Investigations of Growth Factors.
PY - 1999/6
Y1 - 1999/6
N2 - The current study explored whether the neuroprotective effects of vasoactive intestinal peptide (VIP) and its analog Stearyl-Nle17-VIP (SNV) were mediated through cGMP. SNV, was previously found to be 100-fold more potent than VIP in providing neuroprotection. Neuronal survival was assessed in rat cerebral cortical cultures. A cGMP antagonist (RP-8-pCPT-cGMPS, 10-12-10-9 M) reduced the number of surviving neurons (40-60%), this decline was spared in the presence of SNV (10-13 M). A cGMP agonist (Sp-8-pCPT-cGMPS, 10-14-10-8 M) and SNV (10-16-10-8 M) both provided significant neuroprotection against 10-12 M of the cGMP antagonist. Immunoassays indicated that SNV induced increases in cGMP (two-threefold) in these cultures, whereas VIP was 1000-fold less potent. These results implicate cGMP as a second messenger for VIP/SNV-mediated effects on neuronal survival. Copyright (C) 1999 Elsevier Science Inc.
AB - The current study explored whether the neuroprotective effects of vasoactive intestinal peptide (VIP) and its analog Stearyl-Nle17-VIP (SNV) were mediated through cGMP. SNV, was previously found to be 100-fold more potent than VIP in providing neuroprotection. Neuronal survival was assessed in rat cerebral cortical cultures. A cGMP antagonist (RP-8-pCPT-cGMPS, 10-12-10-9 M) reduced the number of surviving neurons (40-60%), this decline was spared in the presence of SNV (10-13 M). A cGMP agonist (Sp-8-pCPT-cGMPS, 10-14-10-8 M) and SNV (10-16-10-8 M) both provided significant neuroprotection against 10-12 M of the cGMP antagonist. Immunoassays indicated that SNV induced increases in cGMP (two-threefold) in these cultures, whereas VIP was 1000-fold less potent. These results implicate cGMP as a second messenger for VIP/SNV-mediated effects on neuronal survival. Copyright (C) 1999 Elsevier Science Inc.
KW - Cyclic guanosine monophosphate (cGMP)
KW - Enzymeimmunoassays (EIA)
KW - Immunocytochemistry
KW - Neuronal survival
KW - Radioimmunoassays (RIA)
KW - Stearyl-Nle-VIP (SNV)
KW - Vasoactive intestinal peptide (VIP)
UR - http://www.scopus.com/inward/record.url?scp=0032871180&partnerID=8YFLogxK
U2 - 10.1016/S0196-9781(99)00017-0
DO - 10.1016/S0196-9781(99)00017-0
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0032871180
SN - 0196-9781
VL - 20
SP - 629
EP - 633
JO - Peptides
JF - Peptides
IS - 5
ER -