Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants

Ohad Roth, Sela Yechezkel, Ori Serero, Avi Eliyahu, Inna Vints, Pan Tzeela, Alberto Carignano, Dorina P. Janacek, Verena Peters, Amit Kessel, Vikas Dwivedi, Mira Carmeli-Weissberg, Felix Shaya, Adi Faigenboim-Doron, Kien Lam Ung, Bjørn Panyella Pedersen, Joseph Riov, Eric Klavins, Corinna Dawid, Ulrich Z. HammesNir Ben-Tal, Richard Napier, Einat Sadot*, Roy Weinstain*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Clonal propagation of plants by induction of adventitious roots (ARs) from stem cuttings is a requisite step in breeding programs. A major barrier exists for propagating valuable plants that naturally have low capacity to form ARs. Due to the central role of auxin in organogenesis, indole-3-butyric acid is often used as part of commercial rooting mixtures, yet many recalcitrant plants do not form ARs in response to this treatment. Here we describe the synthesis and screening of a focused library of synthetic auxin conjugates in Eucalyptus grandis cuttings and identify 4-chlorophenoxyacetic acid–l-tryptophan-OMe as a competent enhancer of adventitious rooting in a number of recalcitrant woody plants, including apple and argan. Comprehensive metabolic and functional analyses reveal that this activity is engendered by prolonged auxin signaling due to initial fast uptake and slow release and clearance of the free auxin 4-chlorophenoxyacetic acid. This work highlights the utility of a slow-release strategy for bioactive compounds for more effective plant growth regulation.

Original languageEnglish
JournalNature Biotechnology
DOIs
StateAccepted/In press - 2024

Funding

FundersFunder number
United States - Israel Binational Agricultural Research and Development FundIS-5195-19R
Purdue University
Deutsche ForschungsgemeinschaftHA3468/6-3, SFB924 TPA08, SFB024 TPB12
Israel Science Foundation1057/21
Tel Aviv University
Ministry of Agriculture and Rural Development20-01-0270, 20-10-0067, 13-37-0005

    Fingerprint

    Dive into the research topics of 'Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants'. Together they form a unique fingerprint.

    Cite this