SKY detection of chromosome rearrangements in two cases of tMDS with a complex karyotype

Ninette Cohen, Luba Trakhtenbrot, Mona Yukla, Yosef Manor, Elena Gaber, Gabi Yosef, Ninette Amariglio, Gideon Rechavi, Aliza Amiel

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we used spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) as complementary techniques for the analysis of two therapy-related secondary myelodysplastic syndrome (t-MDS) cases with complex karyotypes, previously analyzed by G-banding. Different types of SKY's cytogenetic contributions include confirmation of G-banding results, identification of partially characterized rearrangements, identification of marker chromosomes unidentified by G-banding, and detection of cryptic reciprocal translocations. In particular, the ability of SKY to clarify a number of markers led to the comprehension of clonal evolution. The common aberration found in these two t-MDS cases was the fragility of chromosome 5 and monosomy of chromosome 18. We clearly present that the use of SKY combined with conventional G-banding analysis and FISH has assisted in the identification of important chromosomal events that may play a key role in the development of t-MDS.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume138
Issue number2
DOIs
StatePublished - 15 Oct 2002
Externally publishedYes

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