@article{eb9c16c5db6b403ab6c1a721e58cb290,
title = "Skin regeneration with all accessory organs following ablation with irreversible electroporation",
abstract = "Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.",
keywords = "electroporation, extracellular matrix, pulsed electric field, regeneration, scar, scarless, skin",
author = "Alexander Golberg and Martin Villiger and {Felix Broelsch}, G. and Quinn, {Kyle P.} and Hassan Albadawi and Saiqa Khan and Watkins, {Michael T.} and Irene Georgakoudi and Austen, {William G.} and Marianna Bei and Bouma, {Brett E.} and Mihm, {Martin C.} and Yarmush, {Martin L.}",
note = "Publisher Copyright: Copyright {\textcopyright} 2016 John Wiley & Sons, Ltd.",
year = "2018",
month = jan,
doi = "10.1002/term.2374",
language = "אנגלית",
volume = "12",
pages = "98--113",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Inc.",
number = "1",
}