Six-pack holographic imaging for dynamic rejection of out-of-focus objects

Simcha K. Mirsky, Natan T. Shaked*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Six-pack holography is adapted to reject out-of-focus objects in dynamic samples, using a single camera exposure and without any scanning. By illuminating the sample from six different angles in parallel using a low-coherence source, out-of-focus objects are laterally shifted in six different directions when projected onto the focal plane. Then pixel-wise averaging of the six reconstructed images creates a significantly clearer image, with rejection of out-of-focus objects. Dynamic imaging results are shown for swimming microalgae and flowing microbeads, including numerical refocusing by Fresnel propagation. The averaged images reduced the contribution of out-of-focus objects by up to 83% in comparison to standard holograms captured using the same light source, further improving the system sectioning capabilities. Both simulation and experimental results are presented.

Original languageEnglish
Pages (from-to)632-646
Number of pages15
JournalOptics Express
Volume29
Issue number2
DOIs
StatePublished - 18 Jan 2021

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