Site-specific recombination in mammalian cells expressing the int recombinase of bacteriophage HK022: Site-specific recombination in mammalian cells promoted by a phage integrase

Mikhail Kolot, Nava Silberstein, Ezra Yagil

Research output: Contribution to journalArticlepeer-review

Abstract

The int gene of bacteriophage HK022; coding for the integrase protein, was cloned in a mammalian -expression vector downstream of the human cytomegalovirus (CMV) promoter. Green monkey kidney cells (COS-1) and mouse embryo fibroblast cells (NIH3T3) transiently transfected with the recombinant plasmid express the integrase protein. Co-transfection of this plasmid with reporter plasmids for site-specific recombination and PCR analyses show that the integrase promotes site-specific integration as well as excision. These reactions occurred without the need to supply integration host factor and excisionase, the accessory proteins that are required for integrase-promoted site-specific recombination in vitro as well as in the natural host Escherichia coli.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalMolecular Biology Reports
Volume26
Issue number3
DOIs
StatePublished - Aug 1999

Keywords

  • Bacteriophage HK022
  • Integrase
  • Mouse embryo Cells
  • Site-specific recombination

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