Single-Walled Carbon Nanotube Sensor Selection for the Detection of MicroRNA Biomarkers for Acute Myocardial Infarction as a Case Study

Adi Hendler-Neumark, Verena Wulf, Gili Bisker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are single-stranded non-coding short ribonucleic acid sequences that take part in many cellular and biological processes. Recent studies have shown that altered expression of miRNAs is involved in pathological processes, and they can thus be considered biomarkers for the early detection of various diseases. Here, we demonstrate a selection and elimination process of fluorescent single-walled carbon nanotube (SWCNT) sensors for miRNA biomarkers based on RNA-DNA hybridization with a complementary DNA recognition unit bound to the SWCNT surface. We use known miRNA biomarkers for acute myocardial infarction (AMI), commonly known as a heart attack, as a case study. We have selected five possible miRNA biomarkers which are selective and specific to AMI and tested DNA-SWCNT sensor candidates with the target DNA and RNA sequences in different environments. Out of these five miRNA sensors, three could recognize the complementary DNA or RNA sequence in a buffer, showing fluorescence modulation of the SWCNT in response to the target sequence. Out of the three working sensors in buffer, only one could function in serum and was selected for further testing. The chosen sensor, SWCNT-miDNA208a, showed high specificity and selectivity toward the target sequence, with better performance in serum compared to a buffer environment. The SWCNT sensor selection pipeline highlights the importance of testing sensor candidates in the appropriate environment and can be extended to other libraries of biomarkers.

Original languageEnglish
Pages (from-to)3713-3722
Number of pages10
JournalACS Sensors
Volume8
Issue number10
DOIs
StatePublished - 27 Oct 2023

Funding

FundersFunder number
Zimin Institute for Engineering Solutions Advancing Better Lives
European Research Council101039127
Ministry of Science, Technology and Space3-17426
Israel Science Foundation456/18, 196/22
Tel Aviv University
Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering
Ministry of Defense
Israel Innovation Authority
Zimin Institute for Engineering Solutions Advancing Better Lives, Tel Aviv University

    Keywords

    • acute myocardial infarction
    • biosensor
    • fluorescence
    • microRNA
    • single-walled carbon nanotubes

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