Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Gideon Fleminger; Beka Solomon; Tamar Wolf; Eran Hadas

doi:10.1007/BF02921504

Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Gideon Fleminger^*
, Beka Solomon
, Tamar Wolf
, Eran Hadas

^*Corresponding author for this work

Biotechnology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

Original language	English
Pages (from-to)	231-238
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1007/BF02921504
State	Published - Dec 1990

Keywords

Eupergit C
Monoclonal antibodies
carbohydrates
periodate oxidation

Access to Document

10.1007/BF02921504

Cite this

@article{1b51446f4157479e9a7699de6e46ada4,

title = "Single step oxidative binding of antibodies to hydrazide-modified eupergit C",

abstract = "A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.",

keywords = "Eupergit C, Monoclonal antibodies, carbohydrates, periodate oxidation",

author = "Gideon Fleminger and Beka Solomon and Tamar Wolf and Eran Hadas",

year = "1990",

month = dec,

doi = "10.1007/BF02921504",

language = "אנגלית",

volume = "26",

pages = "231--238",

journal = "Applied Biochemistry and Biotechnology",

issn = "0273-2289",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Single step oxidative binding of antibodies to hydrazide-modified eupergit C

AU - Fleminger, Gideon

AU - Solomon, Beka

AU - Wolf, Tamar

AU - Hadas, Eran

PY - 1990/12

Y1 - 1990/12

N2 - A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

AB - A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

KW - Eupergit C

KW - Monoclonal antibodies

KW - carbohydrates

KW - periodate oxidation

UR - https://www.scopus.com/pages/publications/0025641691

U2 - 10.1007/BF02921504

DO - 10.1007/BF02921504

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0025641691

SN - 0273-2289

VL - 26

SP - 231

EP - 238

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 3

ER -

Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this