Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Gideon Fleminger; Beka Solomon; Tamar Wolf; Eran Hadas

doi:10.1007/BF02921504

Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Gideon Fleminger^*, Beka Solomon, Tamar Wolf, Eran Hadas

^*Corresponding author for this work

Biotechnology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

Original language	English
Pages (from-to)	231-238
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1007/BF02921504
State	Published - Dec 1990

Keywords

Eupergit C
Monoclonal antibodies
carbohydrates
periodate oxidation

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10.1007/BF02921504

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abstract = "A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.",

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AU - Solomon, Beka

AU - Wolf, Tamar

AU - Hadas, Eran

PY - 1990/12

Y1 - 1990/12

N2 - A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

AB - A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

KW - Eupergit C

KW - Monoclonal antibodies

KW - carbohydrates

KW - periodate oxidation

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Single step oxidative binding of antibodies to hydrazide-modified eupergit C

Abstract

Keywords

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