TY - JOUR
T1 - Single-molecule approaches for DNA damage detection and repair
T2 - A focus on Repair Assisted Damage Detection (RADD)
AU - Detinis Zur, Tahir
AU - Deek, Jasline
AU - Ebenstein, Yuval
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/9
Y1 - 2023/9
N2 - The human genome is continually exposed to various stressors, which can result in DNA damage, mutations, and diseases. Among the different types of DNA damage, single-strand lesions are commonly induced by external stressors and metabolic processes. Accurate detection and quantification of DNA damage are crucial for understanding repair mechanisms, assessing environmental impacts, and evaluating response to therapy. However, traditional techniques have limitations in sensitivity and the ability to detect multiple types of damage. In recent years, single-molecule fluorescence approaches have emerged as powerful tools for precisely localizing and quantifying DNA damage. Repair Assisted Damage Detection (RADD) is a single-molecule technique that employs specific repair enzymes to excise damaged bases and incorporates fluorescently labeled nucleotides to visualize the damage. This technique provides valuable insights into repair efficiency and sequence-specific damage. In this review, we discuss the principles and applications of RADD assays, highlighting their potential for enhancing our understanding of DNA damage and repair processes.
AB - The human genome is continually exposed to various stressors, which can result in DNA damage, mutations, and diseases. Among the different types of DNA damage, single-strand lesions are commonly induced by external stressors and metabolic processes. Accurate detection and quantification of DNA damage are crucial for understanding repair mechanisms, assessing environmental impacts, and evaluating response to therapy. However, traditional techniques have limitations in sensitivity and the ability to detect multiple types of damage. In recent years, single-molecule fluorescence approaches have emerged as powerful tools for precisely localizing and quantifying DNA damage. Repair Assisted Damage Detection (RADD) is a single-molecule technique that employs specific repair enzymes to excise damaged bases and incorporates fluorescently labeled nucleotides to visualize the damage. This technique provides valuable insights into repair efficiency and sequence-specific damage. In this review, we discuss the principles and applications of RADD assays, highlighting their potential for enhancing our understanding of DNA damage and repair processes.
KW - DNA damage
KW - DNA repair
KW - Damage quantification
KW - Fluorescence microscopy
KW - Optical genomic mapping
KW - Repair Assisted Damage Detection (RADD)
KW - Repair dynamics
KW - Repair enzymes
KW - Single-molecule
KW - Single-strand DNA damage
UR - http://www.scopus.com/inward/record.url?scp=85165972124&partnerID=8YFLogxK
U2 - 10.1016/j.dnarep.2023.103533
DO - 10.1016/j.dnarep.2023.103533
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C2 - 37467630
AN - SCOPUS:85165972124
SN - 1568-7864
VL - 129
JO - DNA Repair
JF - DNA Repair
M1 - 103533
ER -