Single Fluorescent Peptide Nanodots

Nadezda Lapshina, Jonathan Jeffet, Gil Rosenman, Yuval Ebenstein, Tal Ellenbogen

Research output: Contribution to journalArticlepeer-review

Abstract

Fluorescent peptide nanodots (PNDs) are bioorganic nanoparticles self-assembled from peptide biomolecules of different origin and complexity. These recently discovered nanodots of biological origin are highly promising for biomedical imaging applications due to their biocompatibility, bright and tunable fluorescence over the entire visible range and photostability. Here we apply single-particle microscopy methods to study the photophysical properties of individual PNDs. We show that the fluorescence spectrum tunability, studied previously only for PND ensembles in solutions, origins at the single-particle level. Temporal dynamics measurements of the single particles reveal fluorescence lifetime in the range of nanoseconds and pronounced fluorescence blinking with continuous bright states of seconds. The latter provides a first evidence of quantum emitter transitions between two states (ON and OFF) in fluorescent PNDs. All these findings advance the understanding of the fluorescence mechanism of PNDs and provide strong motivation for using PNDs as fluorescent agents for various bioimaging and super-resolution techniques.

Original languageEnglish
Pages (from-to)1626-1631
Number of pages6
JournalACS Photonics
Volume6
Issue number7
DOIs
StatePublished - 17 Jul 2019

Keywords

  • bioimaging
  • blinking
  • fluorescence
  • lifetime
  • peptide nanodots
  • single particle spectroscopy

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