Single chain antibodies specific for fatty acids derived from a semi-synthetic phage display library

Ari Gargir, Itzhak Ofek*, Shiri Meron-Sudai, Meital Gal Tanamy, Panagiotis S. Kabouridis, Ahuva Nissim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The biological activities of many acylated molecules are lipid dependent. Lipids, however, are poorly immunogenic or non-immunogenic. We employed a phage display semi-synthetic human antibody library to isolate anti-lipid antibodies. Selection was done against methyl palmitate, a 16 carbon aliphatic chain, and a major component of bacterial glycolipids and lipoproteins in animal cells. The selected single chain variable fragment (scFv) bound specifically to a 16 carbon aliphatic chain and to a lesser extent to a 14 or 18 carbon aliphatic chain and poorly to either 12, 22 or 8 carbon aliphatic chains. Furthermore, the scFv prevented micelle formation of lipoteichoic acid from Gram-positive bacteria; inhibited lipopolysaccharide-induced tumor necrosis factor α release in mononuclear cells; bound to hydrophobic bacterial surfaces, especially those of Gram-positive bacteria, and bound to Lck, a mammalian palmitated lipoprotein. Our data suggest that the phage antibody library can be successfully employed to obtain human anti-aliphatic scFv human antibody fragment with potential therapeutic applications in neutralizing the deleterious effects of bacterial toxins as well as in structure-function analysis of lipoproteins in animal cells.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1569
Issue number1-3
DOIs
StatePublished - 15 Jan 2002

Funding

FundersFunder number
Kurt Leon Foundation
Leweinstein Foundation

    Keywords

    • Lipoteichoic acid
    • Methyl palmitate
    • Phage display
    • Single chain variable fragment

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