Single and Multiple Quantum NMR Relaxation Times of Sodium and Potassium in Red Blood Cells

Hadassah Shinar*, Uzi Eliav, Gil Navon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The two components T2f and T2s of the transverse magnetization decay curve of 23Na in dog erythrocytes were measured using the triple quantum filtered spectra. It was shown that this technique, in spite of its inferior sensitivity, may be used for systems where T2f and T2s are not very different. Thus it was possible to measure the effect of 2, 3‐diphosphoglycerate (2, 3 DPG) depletion on the intracellular 23Na relaxation rates. The effect found indicates some contribution of hemoglobin‐2, 3‐DPG complexes to the 23Na relaxation in intact erythrocytes. 39K relaxation times were measured in human and chicken erythrocytes. The results indicate binding similar to that of 23Na. Finally, a decrease of the cellular volume of erythrocytes was found to shorten 23Na relaxation times. The result was interpreted as due to an increase in intracellular concentration of proteins.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalIsrael Journal of Chemistry
Volume32
Issue number2-3
DOIs
StatePublished - 1992

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