Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells

Yoav N. Nygate, Gyanendra Singh, Itay Barnea, Natan T. Shaked

Research output: Contribution to journalArticlepeer-review

Abstract

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.

Original languageEnglish
Pages (from-to)2587-2590
Number of pages4
JournalOptics Letters
Volume43
Issue number11
DOIs
StatePublished - 1 Jun 2018

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