Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Dmitry Torchinsky, Yael Michaeli, Natalie R. Gassman, Yuval Ebenstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.

Original languageEnglish
Pages (from-to)11414-11417
Number of pages4
JournalChemical Communications
Volume55
Issue number76
DOIs
StatePublished - 2019

Funding

FundersFunder number
BeyondSeq consortium
European Research Council Proof of Concept767931
Joint Israeli German R&D nanotechnology, Israel Innovation Authority61976
US National Institute of Health
National Institute of Environmental Health SciencesR21ES028015
European Commission634890
European Research Council337830

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