Simplified detection of eosinophils in induced sputum

Elizabeth Fireman, Brenda Toledano, Nina Buchner, Moshe Stark, Yehuda Schwarz

Research output: Contribution to journalArticlepeer-review

Abstract

Objective and design: The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler modification of the process for identifying eosinophilic inflammation from induced sputum (IS) samples. Material: Samples of 81 patients referred for IS assessment of respiratory diseases were studied. Four different assessment approaches were studied in comparison with the conventional method of selecting plugs proposed by the European Respiratory Society/American Thoracic Society task force. Results: One modification of the conventional method of IS processing emerged as simpler to execute and less technologically demanding than the original one (13.0 ± 18.2 vs. 15.4 ± 22.4% eosinophils, P ≥ 0.19). Conclusion: The simpler approach should now encourage the use of IS as a convenient procedure for evaluating eosinophilic inflammation in less sophisticated laboratories.

Original languageEnglish
Pages (from-to)745-750
Number of pages6
JournalInflammation Research
Volume60
Issue number8
DOIs
StatePublished - Aug 2011

Keywords

  • Asthma
  • Eosinophilic inflammation
  • Induced sputum
  • Non-dedicated laboratories

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