TY - JOUR
T1 - Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in developing tobacco leaves
AU - Oparka, K. J.
AU - Roberts, A. G.
AU - Boevink, P.
AU - Cruz, S. S.
AU - Roberts, I.
AU - Pradel, K. S.
AU - Imlau, A.
AU - Kotlizky, G.
AU - Sauer, N.
AU - Epel, B.
N1 - Funding Information:
This work was supported by the Scottish Office Agriculture, Environment and Fisheries Department (SOAEFD to K. J. O.), the Binational Agricultural Research Development Fund (BARD IS-2636-95 to B. L. E.), and the Deutsche Forschungsgemeinschaft (SFB47/A3 to N. S.). P. B. was supported by an EMBO fellowship. The authors would like to thank James Carrington (Washington State University, Pullman) for pRTL2, Biao Ding (Oklahoma State University, Stillwater) for pRTL2.GFP, and Marion Longstaff (Axis Genetics Plc., Cambridge) for pDX12.
PY - 1999
Y1 - 1999
N2 - Leaves undergo a sink-source transition during which a physiological change occurs from carbon import to export. In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an M(r) up to 50 kDa could move freely through plasmodesmata. During the sink-source transition, the capacity to traffic proteins decreased substantially and was accompanied by a developmental switch from simple to branched forms of plasmodesmata. Inoculation of sink leaves with a movement protein-defective virus showed that virally expressed GFP, but not viral RNA, was capable of trafficking between sink cells during infection. Contrary to dogma that plasmodesmata have a size exclusion limit below 1 kDa, the data demonstrate that nonspecific 'macromolecular trafficking' is a general feature of simple plasmodesmata in sink leaves.
AB - Leaves undergo a sink-source transition during which a physiological change occurs from carbon import to export. In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an M(r) up to 50 kDa could move freely through plasmodesmata. During the sink-source transition, the capacity to traffic proteins decreased substantially and was accompanied by a developmental switch from simple to branched forms of plasmodesmata. Inoculation of sink leaves with a movement protein-defective virus showed that virally expressed GFP, but not viral RNA, was capable of trafficking between sink cells during infection. Contrary to dogma that plasmodesmata have a size exclusion limit below 1 kDa, the data demonstrate that nonspecific 'macromolecular trafficking' is a general feature of simple plasmodesmata in sink leaves.
UR - http://www.scopus.com/inward/record.url?scp=0033000639&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80786-2
DO - 10.1016/S0092-8674(00)80786-2
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AN - SCOPUS:0033000639
VL - 97
SP - 743
EP - 754
JO - Cell
JF - Cell
SN - 0092-8674
IS - 6
ER -