@article{fb1a431abdc64d1f898887a89aa6fca0,
title = "shRNA-mediated RNA interference as a tool for genetic synthetic lethality screening in mouse embryo fibroblasts",
abstract = "Previously, we demonstrated the establishment of synthetic lethality screening in cultured somatic human cells, or mouse embryo fibroblasts (MEFs), for chemicals or mutant genes synergistically lethal with a mutated gene of interest. Here, we show in MEFs that the usage of RNA interference-based genetic suppressor elements encoding short hairpin RNAs (shRNAs) enables for genetic synthetic lethality screening at a frequency much higher than that achieved before with short truncated sense and antisense RNAs. These findings open up the possibility of using in mammalian cells genome-wide shRNA libraries for genetic synthetic lethality screening at the multi-gene level.",
keywords = "Genetic suppressor elements, RNA interference, Synthetic lethality screen",
author = "Yulia Einav and Reuven Agami and Dan Canaani",
note = "Funding Information: This work was supported by a grant awarded to D.C. by the Israel Science Foundation and by the Orgler Family Fund in Cancer Genetics.",
year = "2005",
month = jan,
day = "3",
doi = "10.1016/j.febslet.2004.11.075",
language = "אנגלית",
volume = "579",
pages = "199--202",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1",
}