shRNA-mediated RNA interference as a tool for genetic synthetic lethality screening in mouse embryo fibroblasts

Yulia Einav, Reuven Agami, Dan Canaani

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Previously, we demonstrated the establishment of synthetic lethality screening in cultured somatic human cells, or mouse embryo fibroblasts (MEFs), for chemicals or mutant genes synergistically lethal with a mutated gene of interest. Here, we show in MEFs that the usage of RNA interference-based genetic suppressor elements encoding short hairpin RNAs (shRNAs) enables for genetic synthetic lethality screening at a frequency much higher than that achieved before with short truncated sense and antisense RNAs. These findings open up the possibility of using in mammalian cells genome-wide shRNA libraries for genetic synthetic lethality screening at the multi-gene level.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalFEBS Letters
Volume579
Issue number1
DOIs
StatePublished - 3 Jan 2005

Funding

FundersFunder number
Israel Science Foundation

    Keywords

    • Genetic suppressor elements
    • RNA interference
    • Synthetic lethality screen

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