TY - JOUR
T1 - Sertoli cell maturation in men with azoospermia of different etiologies
AU - Maymon, Batia Bar Shira
AU - Yogev, Leah
AU - Paz, Gedalia
AU - Kleiman, Sandra E.
AU - Schreiber, Letizia
AU - Botchan, Amnon
AU - Hauser, Ron
AU - Yavetz, Haim
N1 - Funding Information:
Supported by the Leo Mintz Fund of Tel Aviv University.
PY - 2002
Y1 - 2002
N2 - Objective: To evaluate the involvement of Sertoli cell in different spermatogenic disorders. Design: Retrospective case-control study. Setting: Teaching hospital. Patient(s): Azoospermic men who underwent testicular biopsy for sperm recovery in preparation for intracytoplasmic sperm injection. Intervention(s): Testicular biopsy evaluation by quantitative immunohistochemistry for the immature Sertoli cell markers anti-Müllerian hormone and cytokeratin 18 (CK-18). Main Outcome Measure(s): Relative area of immature Sertoli cells in testes with focal spermatogenesis, spermatocyte maturation arrest, or normal spermatogenesis. Result(s): The relative area occupied by immature Sertoli cells, as revealed by anti-Müllerian hormone and CK-18 expression, was highest in the 11 men with focal spermatogenesis. In the group representing normal spermatogenesis (obstructive azoospermia, 6 men) and in the group characterized by spermatocyte maturation arrest (6 men), the areas occupied by anti-Müllerian hormone- and CK-18-positive cells were minimal. Conclusion(s): Different etiologies underlie the spermatogenic disorders reported in this study. In focal spermatogenesis with high anti-Müllerian hormone and CK-18 expression, the spermatogenic impairment is associated with the presence of immature Sertoli cells. The detection of normal mature Sertoli cells in the spermatocyte maturation arrest group indicates that the spermatogenic defect that is accompanied by an impairment of meiosis is intrinsic to the germ line without affecting Sertoli cell differentiation.
AB - Objective: To evaluate the involvement of Sertoli cell in different spermatogenic disorders. Design: Retrospective case-control study. Setting: Teaching hospital. Patient(s): Azoospermic men who underwent testicular biopsy for sperm recovery in preparation for intracytoplasmic sperm injection. Intervention(s): Testicular biopsy evaluation by quantitative immunohistochemistry for the immature Sertoli cell markers anti-Müllerian hormone and cytokeratin 18 (CK-18). Main Outcome Measure(s): Relative area of immature Sertoli cells in testes with focal spermatogenesis, spermatocyte maturation arrest, or normal spermatogenesis. Result(s): The relative area occupied by immature Sertoli cells, as revealed by anti-Müllerian hormone and CK-18 expression, was highest in the 11 men with focal spermatogenesis. In the group representing normal spermatogenesis (obstructive azoospermia, 6 men) and in the group characterized by spermatocyte maturation arrest (6 men), the areas occupied by anti-Müllerian hormone- and CK-18-positive cells were minimal. Conclusion(s): Different etiologies underlie the spermatogenic disorders reported in this study. In focal spermatogenesis with high anti-Müllerian hormone and CK-18 expression, the spermatogenic impairment is associated with the presence of immature Sertoli cells. The detection of normal mature Sertoli cells in the spermatocyte maturation arrest group indicates that the spermatogenic defect that is accompanied by an impairment of meiosis is intrinsic to the germ line without affecting Sertoli cell differentiation.
KW - Anti-Müllerian hormone
KW - Cytokeratin-18
KW - Nonobstructive azoospermia
KW - Pairing impairment
KW - Sertoli cells
UR - http://www.scopus.com/inward/record.url?scp=0036253320&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(02)03060-1
DO - 10.1016/S0015-0282(02)03060-1
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AN - SCOPUS:0036253320
SN - 0015-0282
VL - 77
SP - 904
EP - 909
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -