TY - JOUR
T1 - Sequential removal and reconstitution of subunits β and γ from a membrane-bound Fo·Fl-ATP synthase
AU - Philosoph, Sonia
AU - Khananshvili, Daniel
AU - Gromet-Elhanan, Zippora
PY - 1981/7/30
Y1 - 1981/7/30
N2 - The β and γ subunits of the Fo·Fl-ATP synthase complex of Rhodospirillum, rubrum chromatophores were removed in two consecutive steps. The resulting depleted chromatophores lost all their ATP synthesizing activity but retained 70% of the light-induced proton uptake. ATP synthesis could be restored by reattachment of the isolated β and γ subunits together, but not of either one of them separately. These data suggest that the γ and β subunits are required for the operation of the chromatophore ATP synthase, but do not seem to participate in the light-induced proton uptake.
AB - The β and γ subunits of the Fo·Fl-ATP synthase complex of Rhodospirillum, rubrum chromatophores were removed in two consecutive steps. The resulting depleted chromatophores lost all their ATP synthesizing activity but retained 70% of the light-induced proton uptake. ATP synthesis could be restored by reattachment of the isolated β and γ subunits together, but not of either one of them separately. These data suggest that the γ and β subunits are required for the operation of the chromatophore ATP synthase, but do not seem to participate in the light-induced proton uptake.
UR - https://www.scopus.com/pages/publications/0019891330
U2 - 10.1016/0006-291X(81)91271-7
DO - 10.1016/0006-291X(81)91271-7
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AN - SCOPUS:0019891330
SN - 0006-291X
VL - 101
SP - 384
EP - 389
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -