Sequential removal and reconstitution of subunits β and γ from a membrane-bound Fo·Fl-ATP synthase

Sonia Philosoph*, Daniel Khananshvili, Zippora Gromet-Elhanan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The β and γ subunits of the Fo·Fl-ATP synthase complex of Rhodospirillum, rubrum chromatophores were removed in two consecutive steps. The resulting depleted chromatophores lost all their ATP synthesizing activity but retained 70% of the light-induced proton uptake. ATP synthesis could be restored by reattachment of the isolated β and γ subunits together, but not of either one of them separately. These data suggest that the γ and β subunits are required for the operation of the chromatophore ATP synthase, but do not seem to participate in the light-induced proton uptake.

Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume101
Issue number2
DOIs
StatePublished - 30 Jul 1981
Externally publishedYes

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