Separation of tumor-seeking small lymphocytes and tumor cells using percoll velocity gradients

Tamar Nethanel*, Radslav Kinsky, Neomi Moav, Raya Brown, Maya Ran, Isaac P. Witz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Using the polyoma virus-induced ascitic SEYF-a tumor, we evaluated isopycnic and velocity sedimentation gradients of Percoll as methods for separating tumor-seeking lymphocytes and tumor cells. It was established that the velocity sedimentation method is suitable for separation of small lymphocytes lodging within the SEYF-a tumor. This was confirmed by a serological analysis of the separated SEYF-a cell population. The results of this study strongly support our previously reported data demonstrating the in vivo coating of the tumor cells proper with potentially cytotoxic antibodies.

Original languageEnglish
Pages (from-to)43-56
Number of pages14
JournalJournal of Immunological Methods
Volume41
Issue number1
DOIs
StatePublished - 27 Feb 1981

Fingerprint

Dive into the research topics of 'Separation of tumor-seeking small lymphocytes and tumor cells using percoll velocity gradients'. Together they form a unique fingerprint.

Cite this