Abstract
Using the polyoma virus-induced ascitic SEYF-a tumor, we evaluated isopycnic and velocity sedimentation gradients of Percoll as methods for separating tumor-seeking lymphocytes and tumor cells. It was established that the velocity sedimentation method is suitable for separation of small lymphocytes lodging within the SEYF-a tumor. This was confirmed by a serological analysis of the separated SEYF-a cell population. The results of this study strongly support our previously reported data demonstrating the in vivo coating of the tumor cells proper with potentially cytotoxic antibodies.
Original language | English |
---|---|
Pages (from-to) | 43-56 |
Number of pages | 14 |
Journal | Journal of Immunological Methods |
Volume | 41 |
Issue number | 1 |
DOIs | |
State | Published - 27 Feb 1981 |