Separation of the gluconeogenic and mitochondrial functions of pgc-1α through s6 kinase

Yaniv Lustig, Jorge L. Ruas, Jennifer L. Estall, James C. Lo, Srikripa Devarakonda, Dina Laznik, Jang Hyun Choi, Hiraku Ono, Jesper V. Olsen, Bruce M. Spiegelman

Research output: Contribution to journalArticlepeer-review


PGC-1α is a transcriptional coactivator that powerfully regulates many pathways linked to energy homeostasis. Specifically, PGC-1α controls mitochondrial biogenesis in most tissues but also initiates important tissue-specific functions, including fiber type switching in skeletal muscle and gluconeogenesis and fatty acid oxidation in the liver. We show here that S6 kinase, activated in the liver upon feeding, can phosphorylate PGC-1α directly on two sites within its arginine/serine-rich (RS) domain. This phosphorylation significantly attenuates the ability of PGC-1α to turn on genes of gluconeogenesis in cultured hepatocytes and in vivo, while leaving the functions of PGC-1α as an activator of mitochondrial and fatty acid oxidation genes completely intact. These phosphorylations interfere with the ability of PGC-1α to bind to HNF4α, a transcription factor required for gluconeogenesis, while leaving undisturbed the interactions of PGC-1α with ERRα and PPARα, factors important for mitochondrial biogenesis and fatty acid oxidation. These data illustrate that S6 kinase can modify PGC 1α and thus allow molecular dissection of its functions, providing metabolic flexibility needed for dietary adaptation.

Original languageEnglish
Pages (from-to)1232-1244
Number of pages13
JournalGenes and Development
Issue number12
StatePublished - 15 Jun 2011
Externally publishedYes


  • Gluconeogenesis
  • Liver
  • PGC-1α
  • S6K1


Dive into the research topics of 'Separation of the gluconeogenic and mitochondrial functions of pgc-1α through s6 kinase'. Together they form a unique fingerprint.

Cite this