@article{00e5bd8ed4394e45b854444cbd3df21b,
title = "Separation of induction and expression of tight junction formation mediated by proteinases",
abstract = "The formation of tight junctions can be induced in the human adenocarcinoma cell line HT 29 by treatment with trypsin at 37°C. In contrast, after treatment of the cells with trypsin at low temperature (3°C), no tight junctions were observed. However, abundant formation of tight junctions occurred when cells were treated with trypsin at 3°C, washed with soybean trypsin inhibitor, and subsequently incubated at 37°C. Thus, this protocoi allows for the first time the temporal separation of the induction and assembly of tight junctions.",
keywords = "(Human adenocarcinoma), Cell-cell interaction, Proteinase, Tight junction",
author = "Ahuva Talmon and Esther Cohen and Adelbert Bacher and Yehuda Ben-shaul",
note = "Funding Information: This work was supported by grants from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie.",
year = "1984",
month = jan,
day = "25",
doi = "10.1016/0005-2736(84)90337-7",
language = "אנגלית",
volume = "769",
pages = "505--507",
journal = "BBA - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier B.V.",
number = "2",
}