TY - JOUR
T1 - Separation of 'estrogen induced' protein from phosphoprotein phosphatase activity of immature rat uterus
AU - Kaye, A. M.
AU - Walker, M. D.
AU - Somjen, Dalia
PY - 1975
Y1 - 1975
N2 - Preparations of the 'induced protein' which appears in the rat uterus within 40 min of estradiol administration have recently been reported to contain phosphoprotein phosphatase (phosphoprotein phosphohydrolase, EC 3.1.3.16) activity. It was found that these two proteins distribute differently on ammonium sulfate fractionation of uterine cytosol. Preparative cellulose acetate electrophoresis afforded complete (>99.9%) separation of phosphoprotein phosphatase activity from the induced protein. The specific activity of phosphoprotein phosphatase in uterine cytosol was unchanged 1, 4, 12, or 24 hr after estradiol administration. These results are incompatible with the view that the induced protein mediates estrogen action by virtue of an inherent phosphoprotein phosphatase activity.
AB - Preparations of the 'induced protein' which appears in the rat uterus within 40 min of estradiol administration have recently been reported to contain phosphoprotein phosphatase (phosphoprotein phosphohydrolase, EC 3.1.3.16) activity. It was found that these two proteins distribute differently on ammonium sulfate fractionation of uterine cytosol. Preparative cellulose acetate electrophoresis afforded complete (>99.9%) separation of phosphoprotein phosphatase activity from the induced protein. The specific activity of phosphoprotein phosphatase in uterine cytosol was unchanged 1, 4, 12, or 24 hr after estradiol administration. These results are incompatible with the view that the induced protein mediates estrogen action by virtue of an inherent phosphoprotein phosphatase activity.
UR - http://www.scopus.com/inward/record.url?scp=0016711709&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.7.2631
DO - 10.1073/pnas.72.7.2631
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AN - SCOPUS:0016711709
VL - 72
SP - 2631
EP - 2634
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 7
ER -