@article{0fb95f063bd44f6581fe81a7e987f724,
title = "Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis",
abstract = "Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea-agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of ~1-20-kb size migrate differently in 1 M urea containing agarose gels. The two strands are readily distinguished on Southern blots by ss-specific probes. The different migration of the two strands could be attributed to their different, base composition-dependent conformation impinging on the electrophoretic mobility of the ss molecules. This phenomenon can be exploited for the efficient preparation of strand-specific probes and for the separation of the complementary DNA strands for subsequent analysis, offering a new tool for various cell biological research areas.",
author = "{\'E}va Heged{\"u}s and Endre K{\'o}kai and Alexander Kotlyar and Viktor Dombr{\'a}di and G{\'a}bor Szab{\'o}",
note = "Funding Information: The Hungarian Scientific Research Fund (OTKA) [TO48742, 72762 to G. Sz.]; the Ministry of Public Health ETT [067/2006 to G. Sz.]; Janos Bolyai research fund of the Hungarian Academy of Sciences (to E.K.). Funding for open access charge: OTKA.",
year = "2009",
month = jun,
day = "23",
doi = "10.1093/nar/gkp539",
language = "אנגלית",
volume = "37",
pages = "e112--e112",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "17",
}