@inbook{3f5b7f03b551472e9214818cbdae37a5,
title = "Sensitizing plant protein kinases to specific inhibition by atp-competitive molecules",
abstract = "The highly conserved nature of the protein kinase catalytic domain and the low permeability of plant cell membranes pose a challenge to the development of specific inhibitors that target individual protein kinases in vivo. Here, we describe a chemical-genetic approach to specifically sensitize individual plant kinases to cell-permeable small molecules that do not inhibit wild-type kinases. In this approach, a single amino-acid substitution is introduced in the ATP-binding site of the enzyme enabling specific binding of ATP-competitive molecules. Cell-permeable molecules can then be used to specifically target the sensitized allele in transgenic Arabidopsis thaliana plants that do not express the wild-type form of the kinase. This strategy provides a useful tool for the functional characterization of protein kinases in planta and for the dissection of the signaling pathways in which they are involved.",
keywords = "ATP-competitive molecules, Analog-sensitive, Arabidopsis, Gatekeeper, Kinase activity assay, PP1, Protein kinase, Transgenic plants",
author = "Dor Salomon and Chao Zhang and Shokat, {Kevan M.} and Guido Sessa",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2011.",
year = "2011",
doi = "10.1007/978-1-61779-264-9_10",
language = "אנגלית",
isbn = "9781617792632",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "185--197",
booktitle = "Plant Kinases",
}