Background. House dust mites (HDMs) are important causes of persistent allergic diseases, such as asthma and rhinitis. Various types of mites are found in the house dust of many countries, including Israel. Objective. To evaluate the prevalence of sensitization to various HDMs in patients with perennial allergic rhinitis and asthma Methods. Sensitization of 117 patients with persistent rhinitis who attended the Allergy and Asthma Center in Tel Aviv (Israel) was evaluated by a skin prick test (SPT) using standardized allergenic extracts. The tested mites were Dermatophagoides farinae (DF), Dermatophagoides pteronyssinus (DP), Lepidoglyphus destructor (LD), Blomia tropicalis (BT), Tyrophagus putrescentiae (TP), Acarus siro (AS), Glycyphagus domesticus (GD), Blomia kulagini (BK), and Tetranychus urticae (TU). Results. Most patients (n =95, 81) had a positive SPT to at least one mite extract. The three most frequent positive reactions were to DF (78), DP (75), and, unexpectedly, BT (77). The correlation between DF and DP sensitization was higher than the correlation between DF or DP to BT (r =.78 versus r =.60, p < .05). Six patients had positive skin reactions to at least one mite species other than DF, DP or BT, mainly LD (n =2, 2.1) and BK (n= 4, 3.8). Conclusions. The findings of this study identified the most ubiquitous mites to which Israeli patients with allergic rhinitis were sensitized and indicated the importance of BT as an allergen. Extracts of these mites may provide a more accurate diagnosis and effective treatment of respiratory diseases due to mite allergy in Israel and probably in other countries with similar climes.