TY - JOUR
T1 - Sensitization of resting T cells to autologous natural-killer-cell- mediated lysis by phytohemagglutinin
AU - Baraz, Lea
AU - Kotler, Moshe
AU - Condiotti, Reba
AU - Nagler, Arnon
N1 - Funding Information:
Acknowledgements We thank Dr. E. Yefenof for helpful discussion. This work was supported by a grant from the Israel Academy of Sciences and Humanities (grant no. 546/97) and the Naphtali Foundation. Donations from the Abrams family are greatly appreciated.
PY - 1999
Y1 - 1999
N2 - Natural killer (NK) cells are non-T, non-B cell lymphocytes that lyse a variety of tumor and virus-infected cells. In this study, we demonstrated that phytohemagglutinin (PHA) rendered resistant autologous T cells extremely sensitive to natural-killer(NK)-cell-mediated lysis. The sensitization was very rapid and concentration-dependent (0.01-1 μg/ml); 62% and 95% of autologous T cells were lysed by interleukin-2-activated N K cells 5 min and 18 h respectively after treatment with PHA (1 μg/ml). The maximal decrease in the level of MHC class I molecules observed on T cells was 22%. Induction of susceptibility to NK-mediated lysis was correlated with the expression of activation markers on T cells treated for relatively long intervals (more than 18 h) with high concentrations of PHA (more than 0.1 μg/ml). Sensitization of T cells required RNA and protein synthesis, although DNA synthesis was not essential. We propose that this unique system is suitable for studying the mechanisms involved in recognition and killing of target cells by NK cells.
AB - Natural killer (NK) cells are non-T, non-B cell lymphocytes that lyse a variety of tumor and virus-infected cells. In this study, we demonstrated that phytohemagglutinin (PHA) rendered resistant autologous T cells extremely sensitive to natural-killer(NK)-cell-mediated lysis. The sensitization was very rapid and concentration-dependent (0.01-1 μg/ml); 62% and 95% of autologous T cells were lysed by interleukin-2-activated N K cells 5 min and 18 h respectively after treatment with PHA (1 μg/ml). The maximal decrease in the level of MHC class I molecules observed on T cells was 22%. Induction of susceptibility to NK-mediated lysis was correlated with the expression of activation markers on T cells treated for relatively long intervals (more than 18 h) with high concentrations of PHA (more than 0.1 μg/ml). Sensitization of T cells required RNA and protein synthesis, although DNA synthesis was not essential. We propose that this unique system is suitable for studying the mechanisms involved in recognition and killing of target cells by NK cells.
UR - http://www.scopus.com/inward/record.url?scp=0032696224&partnerID=8YFLogxK
U2 - 10.1007/s002620050599
DO - 10.1007/s002620050599
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C2 - 10602888
AN - SCOPUS:0032696224
VL - 48
SP - 507
EP - 516
JO - Cancer Immunology, Immunotherapy
JF - Cancer Immunology, Immunotherapy
SN - 0340-7004
IS - 9
ER -
