Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1

Moria Barlev-Gross, Shay Weiss, Nir Paran, Yfat Yahalom-Ronen, Ofir Israeli, Ital Nemet, Limor Kliker, Neta Zuckerman, Itai Glinert, Tal Noy-Porat, Ron Alcalay, Ronit Rosenfeld, Haim Levy, Ohad Mazor, Michal Mandelboim, Ella Mendelson, Adi Beth-Din, Tomer Israely, Adva Mechaly*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region - results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.

Original languageEnglish
Pages (from-to)616-619
Number of pages4
JournalJournal of Infectious Diseases
Volume224
Issue number4
DOIs
StatePublished - 15 Aug 2021

Keywords

  • 501Y.V1
  • 501Y.V2
  • Antibodies
  • Antigen detection
  • SARS-CoV-2
  • Variants of Concern
  • spike protein

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