Sensing of UO22+ and design of logic gates by the application of supramolecular constructs of ion-dependent DNAzymes

Michal Moshe, Johann Elbaz, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Supramolecular constructs composed of ion-dependent DNAzymes and their substrates were used to develop DNAzyme cascades that enabled the sensitive detection of UO22+ or the activation of logic gate operations. The supramolecular complex between the UO22+ -dependent DNAzyme and its substrate leads, in the presence of UO 22+, to the cleavage of the substrate and to the release of the HRP-mimicking DNAzyme that enables the optical analysis of UO 22+ (detection limit 1 × 10-9 M). Similarly, supramolecular complexes between the Mg2+ - and UO 22+ -dependent DNAzymes and tailored substrates enables the design of the "OR" and "AND" logic gates, using Mg 2+ and UO22+ as inputs.

Original languageEnglish
Pages (from-to)1196-1200
Number of pages5
JournalNano Letters
Volume9
Issue number3
DOIs
StatePublished - 11 Mar 2009
Externally publishedYes

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