Self-Assembled Peptide Nano-Superstructure towards Enzyme Mimicking Hydrolysis

Yu Chen, Yuqin Yang, Asuka A. Orr, Pandeeswar Makam, Boris Redko, Elvira Haimov, Yannan Wang, Linda J.W. Shimon, Sigal Rencus-Lazar, Meiting Ju, Phanourios Tamamis*, Hao Dong*, Ehud Gazit*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

The structural arrangement of amino acid residues in native enzymes underlies their remarkable catalytic properties, thus providing a notable point of reference for designing potent yet simple biomimetic catalysts. Herein, we describe a minimalistic approach to construct a dipeptide-based nano-superstructure with enzyme-like activity. The self-assembled biocatalyst comprises one peptide as a single building block, readily synthesized from histidine. Through coordination with zinc ion, the peptide self-assembly procedure allows the formation of supramolecular β-sheet ordered nanocrystals, which can be used as basic units to further construct higher-order superstructure. As a result, remarkable hydrolysis activity and enduring stability are demonstrated. Our work exemplifies the use of a bioinspired supramolecular assembly approach to develop next-generation biocatalysts for biotechnological applications.

Original languageEnglish
Pages (from-to)17164-17170
Number of pages7
JournalAngewandte Chemie - International Edition
Volume60
Issue number31
DOIs
StatePublished - 26 Jul 2021

Funding

FundersFunder number
Joint NSFC‐ISF3145/19
Leeor Kronik
Mariana Hildebrand
NSFC-ISF
Texas A and M University
Center for High Performance Computing
European Research Council
National Natural Science Foundation of China21833002
Tel Aviv University
Natural Science Foundation of Jiangsu ProvinceBK20190056
Horizon 2020694426
Nanjing University

    Keywords

    • biocatalysis
    • nano-superstructure
    • peptide
    • self-assembly
    • supramolecular chemistry

    Fingerprint

    Dive into the research topics of 'Self-Assembled Peptide Nano-Superstructure towards Enzyme Mimicking Hydrolysis'. Together they form a unique fingerprint.

    Cite this