Selective medium for isolation of Mycoleptodiscus terrestris from soil sediments of aquatic environments

B. Sneh, J. Stack

Research output: Contribution to journalArticlepeer-review

Abstract

A selective medium was developed for the dilution plate isolation of Mycoleptodiscus terrestris from natural soils and sediments from aquatic environments. The ingredients per liter of the selective medium are as follows: KH2PO4, 0.5 g; MgSO4 · 7H2O, 0.5 g; dextrose, 10.0 g; peptone, 5.0 g; chloramphenicol, 0.25 g; rose bengal, 50 mg; oxgall, 5.0 g; Terraclor (pentachloronitrobenzene, 75% active ingredients), 0.5 g; agar, 15.0 g. After autoclaving, the following ingredients were aseptically added: sorbic acid (0.7% autoclave-sterilized aqueous solution), 5.0 ml; Subdue (25.1% emulsion of metalaxyl), 0.5 ml; Truban (40.7% suspension of etridiazol), 0.05 ml. The colony-restrictive properties of this medium enabled its use in the drop plate method, originally developed for viable counts of bacteria. Alfalfa sprouts as baits were not suitable for quantitative recovery of the fungus, although 5% of alfalfa sprouts were infected with M. terrestris when incubated on soil containing 1.5 x 102 CFU/g.

Original languageEnglish
Pages (from-to)3273-3277
Number of pages5
JournalApplied and Environmental Microbiology
Volume56
Issue number11
DOIs
StatePublished - 1990
Externally publishedYes

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