@article{c874bd111ebf46c3bce7442b3314fa22,
title = "Selective and universal primers for trematode barcoding in freshwater snails",
abstract = "Trematodes are significant pathogens of high medical, veterinary, and environmental importance. They are hard to isolate from their intermediate hosts, and their early life stages are difficult to identify morphologically. Therefore, primers were developed for trematodes to create a species barcoding system and allow selective PCR amplification in mixed samples. The specific oligonucleotide primer was universal for trematodes that infected several freshwater snail species in Israel. The diagnostic tool is based on the 18S rDNA gene. In contrast to morphological identification, trematode barcoding is rapid as it is based on a sequence of only 800 bp, and it classifies species accurately due to high polymorphism between conserved areas.",
keywords = "18S rDNA, Centrocestus formosanus, Collyriclum faba, Flukes, Haplorchis pumilio, Haplorchis taichui, Lecithodendriidae sp, Lissorchus kritskyi, Opecoelidae sp, Paratanaisia bragai, Philophthalmus sp, Pleurogenes claviger, Sanguinicola cf. inermis, Transversotrema haasi",
author = "J. Routtu and D. Grunberg and R. Izhar and Y. Dagan and Y. Guttel and M. Ucko and F. Ben-Ami",
note = "Funding Information: Acknowledgments We thank Yasmin Gabay for technical support and Liron Goren for editing the trematode picture. This research was supported in part by grant no. 2011011 from the United States-Israel Binational Science Foundation (BSF) to FBA and by a grant from the Israel Taxonomy Initiative to FBA and MU.",
year = "2014",
month = jul,
doi = "10.1007/s00436-014-3903-z",
language = "אנגלית",
volume = "113",
pages = "2535--2540",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "7",
}