SEE locomotor behavior test discriminates C57BL/6J and DBA/2J mouse inbred strains across laboratories and protocol conditions

Neri Kafkafi, Cheryl L. Mayo, Gregory I. Elmer, Dina Lipkind, Yoav Benjamini, Ilan Golani

Research output: Contribution to journalArticlepeer-review

Abstract

Conventional tests of behavioral phenotyping frequently have difficulties differentiating certain genotypes and replicating these differences across laboratories and protocol conditions. This study explores the hypothesis that automated tests can be designed to quantify ethologically relevant behavior patterns that more readily characterize heritable and replicable phenotypes. It used SEE (Strategy for the Exploration of Exploration) to phenotype the locomotor behavior of the C57BL/6 and DBA/2 mouse inbred strains across 3 laboratories. The 2 genotypes differed in 15 different measures of behavior, none of which had a significant genotype-laboratory interaction. Within the same laboratory, most of these differences were replicated in additional experiments despite the test photoperiod phase being changed and saline being injected. Results suggest that well-designed tests may considerably enhance replicability across laboratories.

Original languageEnglish
Pages (from-to)464-477
Number of pages14
JournalBehavioral Neuroscience
Volume117
Issue number3
DOIs
StatePublished - Jun 2003

Fingerprint

Dive into the research topics of 'SEE locomotor behavior test discriminates C57BL/6J and DBA/2J mouse inbred strains across laboratories and protocol conditions'. Together they form a unique fingerprint.

Cite this